Taq dna polymerase

It is frequently used in the polymerase chain reaction (PCR), a method for . It has a DNA polymerase activity and a exonuclease activity. The Polymerase Chain Reaction (PCR) is a powerful and sensitive technique for DNA amplification (1). These guidelines cover routine PCR. Find product specific information including CAS, MSDS, protocols and references.

Het enzym is geïsoleerd van Thermus aquaticus en heeft een molecuulmassa van ongeveer kDa. Hot start is een bekende, handige methode om de specificiteit van PCR te verbeteren. Aan conventionele technologieën, zoals antilichaam inhibitie of chemische blokkering van DNA -polymerases, zijn enkele beperkingen verbonden: de activering kan in eerste instantie veel tijd kosten, de prestatie van het . PCR can be initiated from as few as primed templates.

In addition, CoralLoad PCR Buffer (containing two gel-tracking dyes) is also . The accuracy of Pfu DNA Polymerase has been reported to be 7. Park Y(1), Choi H, Lee DS, Kim Y. It is suitable for applications requiring high temperature synthesis of DNA. Our real-time qPCR and cDNA synthesis reagents set the standard for assay reproducibility, specificity and sensitivity. Stable thermophilic DNA polymerase, suitable for applications requiring high temperature synthesis of DNA.

Taq DNA polymerase from. Ultrapure, recombinant protein. It can be used for routine PCR applications including . A versatile thermostable polymerase that can be used for a variety of PCR applications. Online vertaalwoordenboek. This recombinant thermostable DNA polymerase expressed from a sequence originating from the thermophilic bacterium Thermus aquaticus can be used for any standard PCR application and is compatible with all standard and fast cycling real-time instruments.

Like native Taq, it polymerizes DNA from a primer annealed to a DNA template in the presence of deoxyribonucleotide triphosphates. Supplied with a 10x NHReaction buffer containing 1. M MgCI(final concentration). Er is geen informatie beschikbaar voor deze pagina.

The method used ammonium sulfate to precipitate the enzyme, and it saved effort and money but not time. TAQ: STRUCTURE OF TAQ DNA POLYMERASE. The molecular weight of the recombinant protein is 94kD. This makes it very suitable for standard applications.

The error rate of this Taq . Strong red color of the enzyme allows user to check the polymerase addition. Optimize routine PCR with this highly thermostable DNA polymerase. Modern diagnostics, molecular biology and genetic engineering need DNA polymerases which demonstrate improved performance.